“It is a good idea to sample your colonies regularly throughout the year using the sugar shake, alcohol wash, or sticky board method. This will indicate mite load and the
necessity for Varroa treatment. Treatment is recommended if the sugar shake or alcohol wash results in 3 mites per 100 bees or if there are more than 30 mites per day
on the sticky board.”

USDA Agriculture Research Service, Carl Hayden Bee Research Center: Tucson, AZ